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Quantification of the optical properties of two-layered turbid media by simultaneously analyzing the spectral and spatial information of steady-state diffuse reflectance spectroscopy

机译:通过同时分析稳态漫反射光谱的光谱和空间信息来量化两层混浊介质的光学性质

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摘要

We applied hyperspectral imaging to measure spatially-resolved diffuse reflectance spectra in the visible range and an iterative inversion method based on forward Monte Carlo modeling to quantify optical properties of two-layered tissue models. We validated the inversion method using spectra experimentally measured from liquid tissue mimicking phantoms with known optical properties. Results of fitting simulated data showed that simultaneously considering the spatial and spectral information in the inversion process improves the accuracies of estimating the optical properties and the top layer thickness in comparison to methods fitting reflectance spectra measured with a single source-detector separation or fitting spatially-resolved reflectance at a single wavelength. Further development of the method could improve noninvasive assessment of physiological status and pathological conditions of stratified squamous epithelium and superficial stroma.
机译:我们应用高光谱成像技术来测量可见光范围内的空间分辨漫反射光谱,并基于前向蒙特卡洛建模的迭代反演方法来量化两层组织模型的光学特性。我们使用从液态组织模仿幻象的具有已知光学特性的实验测量光谱验证了反演方法。拟合模拟数据的结果表明,与通过单源探测器分离测量反射光谱或在空间上进行光谱拟合的方法相比,在反演过程中同时考虑空间和光谱信息可提高估计光学特性和顶层厚度的准确性。在单个波长下的分辨反射率。该方法的进一步发展可以改善对鳞状上皮和浅表基质的生理状态和病理状况的无创评估。

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